Acta Anatomica Sinica

›› 2009, Vol. 40 ›› Issue (4): 680-684.doi: 10.3969/j.issn.0529-1356.2009.04.032

• 技术方法 • Previous Articles     Next Articles

Compararative investigation of proliferation of lymphocytes stimulated by human umbilical cord blood mesenchymal stem cells with CFSE and MTT

  

  1. 1. Department of Histology and Embryology, Capital Medical University, Beijing 100069, China; BR>2. Beijing Institute for Neuroscience Capital Medical Unviersity, Beijing 100069, China； 3. China Rehabilitation Reserch Center Medical Laboratory, Beijing 100068, China BR>
  • Received:2008-04-23 Revised:2008-06-18 Online:2009-08-06
  • Contact: JI Feng-qing1

Abstract: Objective To compare the sensitivities of the stimulation effect of human cord blood mesenchymal stem cells(CB-MSCs) and CB-MSCs of neuronal differentiation to lymphocytes(LCs) detected with carboxyfluorescein didcetate(CFSE) and MTT. Methods To prepare LCs from SD rat and divided into four group stimulating cells: 1. CB-MSCs; 2. Dif-CB-HSCs; 3. SH-SY5Y（positive control）; 4. Auto-LC（negative control）.Stimulating cells were respectively Co-cultured with LCs. The proliferation of LCs was detected with MTT and CFSE (EM>n/EM>=3). Results CB-MSCs and Dif-CB-HSCs stimulated LCs to proliferate more weakly than positive control detected with MTT and CFSE. The quantity of proliferation of lymphocytes Co-cultured with CB-MSCs and Dif-CB-HSCs were higher than that of Auto-LC detected with CFSE. But MTT OD value of CB-MSCs and Dif-CB-HSCs was a little lower than that of Auto-LC. Statistical analysis results showed no significant difference.Conclusion CFSE can reflect proliferation status of lymphocytes better than MTT. CFSE shows mor

Key words: Umbilical cord blood mesenchymal stem cell, Lymphocyte, Proliferation, Carboxyfluorescein didcetate, MTT assay

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